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If you are looking for TCBS Agar, developed by Kobayashi et al, is designed for Vibrio isolation, recommended by APHA for V. cholerae and V. parahaemolyticus. With its selective properties enhanced by sodium cholate, it allows the growth of diverse Vibrio strains, each displaying distinct colony morphologies. V. cholerae produces yellow colonies due to sucrose fermentation, while V. parahaemolyticus forms blue-green colonies. Although highly selective for Vibrio, occasional Pseudomonas and Aeromonas isolates may appear. H2S-negative colonies are indicative of Vibrio. Inoculating heavily with fecal specimens is crucial, overcoming potential inhibitions from sucrose fermentation.
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