01/06/2024 Healthcare
Thiosulfate-citrate-bile salts-sucrose (TCBS) agar is a selective and differential medium primarily used for isolating and identifying Vibrio species, including Vibrio cholerae and Vibrio parahaemolyticus. The medium contains bile salts to inhibit non-Vibrio bacteria, sucrose for fermentation differentiation, and pH indicators that cause Vibrio colonies to appear yellow (sucrose fermenters) or green/blue-green (non-sucrose fermenters). TCBS agar's high pH enhances the selectivity for Vibrio species, making it an essential tool in clinical and environmental microbiology.
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