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Silica gel column chromatography depends on the activity of the silica gel as an adsorbent and polarity of the mixture. With low polarity of the mixture and strong adsorbent activity, it provides highly effective separation of the compound.

But at the other end, if there is high polarity of the mixture and high activity of adsorbent, the separation result would not be up to the mark. Silica gel for the separation is used in different methods of chromatography. They include analytical, preparative, process and gravity chromatography.

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