Many genetic manipulations are limited by difficulty in obtaining adequate levels of protein expression. Bioinformatic and experimental studies have identified nucleotide sequence features that may increase expression.It is difficult to assess the relative influence of these features.
Glycation of proteins, nucleotides and basic phospholipids by glyoxal and methylgliyoxal-physiological subtrates of glyoxalase 1- is potentially damaging to the proteome , genome and lipidome.
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